Journal:
Article Title: PhaG-Mediated Synthesis of Poly(3-Hydroxyalkanoates) Consisting of Medium-Chain-Length Constituents from Nonrelated Carbon Sources in Recombinant Pseudomonas fragi
doi:
Figure Lengend Snippet: Bacterial strains and plasmids used in these studies
Article Snippet: TABLE 1 Strain or plasmid Relevant characteristic(s) Source or reference Strains P. putida GPp104 PHA synthase-negative mutant of P. putida KT2442 (mt-2, hsdR1 [r − m + ]) without TOL plasmid 5 PHAG N -21 PhaG-negative mutant of P. putida KT2440 15 P. fragi Wild type DSM 3456 P. oleovorans OCT plasmid ATCC 29347 E. coli S17-1 proA thi-1 recA ; harbors the tra genes of plasmid RP4 in the chromosome 19 JM109 recA1 endA1 gyrA96 thi-1 hsdR17 (r k − , m k + ) supE44 relA1 λ − lac [F′ proAB lacI q ZΔM15] 17 RS3097 e14 − ( mcrA − ) fadR41 ( ts ) zcg-101 ::Tn 10 20 Plasmids pBHR71 pBluescript SK(−) containing phaC1 from P. aeruginosa downstream of lac promoter 8 pBHR75 pUCP27 containing the 1.3-kbp Bam HI- Hin dIII fragment comprising phaG , including the native promoter 15 pBBR1MCS-2 Km r , broad host range, lacPOZ ′ 7 pBHR81 pBBR1MCS-2 containing coding region of phaG downstream of lac promoter 15 pSF2 pBBR1MCS-2 containing coding region of phaC1 gene from P. aeruginosa downstream of lac promoter This study pJRDEE32 pJRD215 containing the phaC and phaJ genes of A. caviae 3 pPS2 pBBR1MCS-2 containing coding region of phaC gene from A. caviae downstream of lac promoter This study pBHR86 pBBR1MCS-2 containing coding region of phaC1 gene from P. aeruginosa downstream of lac promoter and coding region of phaG from P. putida downstream of phaC1 , including the native promoter This study Open in a separate window Bacterial strains and plasmids used in these studies . .
Techniques: Plasmid Preparation, Mutagenesis
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